Product-based kinetic analysis reveals limitations of substrate depletion method for assessing bile salt hydrolase activity
Researchers have developed an LC-MS-based assay to better characterize bile salt hydrolase (BSH) activity in the microbiome, revealing that substrate depletion methods are often unreliable. The study suggests that Vmax-oriented analysis provides a more accurate assessment of enzymatic activity in murine models.
Why it matters
Improving the accuracy of BSH activity measurement is critical for understanding host-microbiome interactions and developing new pharmaceutical treatments.
Scientific Reports ( 2026 ) Cite this article
We are providing an unedited version of this manuscript to give early access to its findings. Before final publication, the manuscript will undergo further editing. Please note there may be errors present which affect the content, and all legal disclaimers apply.
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