Glowing Reporter Opens Agroinfiltration to More Plant Species

FBP precursor caffeic acid is present in land plant lineages. (A) Phylogenetic tree of the algae and 10 land plant species used in this study. Species marked with gray dots correspond to lateral branching. The number represents the potential number of caffeic acid synthesis related genes in each species. PAL, phenylalanine ammonia-lyase; C4H, cinnamic acid 4-hydroxylase; 4CL, 4-hydroxycinnamate: CoA ligase; HCT, 4-hydroxycinnamoyl CoA: shikimate/quinate hydroxycinnamoyl transferase; C3′H, 4-coumaroyl shikimate/quinate 3′-hydroxylase; CSE, caffeoyl shikimate esterase; C3H, coumarate 3-hydroxylase. Chr, C. reinhardtii; Map, M. polymorpha; Php, P. patens; Dic, D. complanatum; Cer, C. richardii; Gib, G. biloba; Amt, A. trichopoda; Nyc, N. colorata; Ors, O. sativa; Sol, S. lycopersicum; Art, A. thaliana. (B) Integrated the pathway of plant caffeic acid biosynthesis into the FBP cycle. The black arrows represent direct biochemical reactions, and FBP key genes are highlighted with the green background.