D&D‑seq Uses Base Editing to Map DNA-Protein Interactions in Single Cells
Researchers have developed D&D-seq, a new molecular technique that uses base-editing enzymes to map DNA-protein interactions in single cells. This method allows for the detection of transient or weak regulatory interactions that traditional sequencing methods often miss.
Why it matters
This advancement provides a more precise tool for understanding gene regulation, which is critical for research into diseases like leukemia.
A new molecular recording strategy is giving researchers a way to capture DNA–protein interactions in single cells, including the weak and transient contacts that shape gene regulation but often slip past existing assays. The method, called D&D‑seq (docking and deamination followed by sequencing), layers a base‑editing enzyme onto an antibody‑binding nanobody, turning fleeting interactions into durable sequence marks.
The article is a technical summary of scientific research published in a peer-reviewed journal.
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